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The Journal of Medical Microbiology, Vol 29, Issue 4 255-261, Copyright © 1989 by Society for General Microbiology

JOURNAL ARTICLE

A comparison of immunoblot and DNA restriction patterns in characterising methicillin-resistant isolates of Staphylococcus aureus

J. P. Burnie, R. C. Matthews, W. Lee and D. Murdoch
Department of Medical Microbiology, St Bartholomew's Hospital, London.

The ability of EcoR1 restriction enzyme fragmentation patterns and of immunoblotting to differentiate methicillin-resistant isolates of Staphylococcus aureus were compared. All isolates examined were typable by both methods and the reproducibility of each was excellent. Immunoblotting differentiated eight types and DNA restriction patterns four. The former technique was of value in characterising methicillin-resistant isolates of S. aureus and controlling an outbreak due to them.


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J. P. Burnie, R. C. Matthews, T. Carter, E. Beaulieu, M. Donohoe, C. Chapman, P. Williamson, and S. J. Hodgetts
Identification of an Immunodominant ABC Transporter in Methicillin-Resistant Staphylococcus aureus Infections
Infect. Immun., June 1, 2000; 68(6): 3200 - 3209.
[Abstract] [Full Text] [PDF]


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