HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Magee, J. T. Articles by Aries, R. E. Search for Related Content PubMed PubMed Citation Articles by Magee, J. T. Articles by Aries, R. E. Agricola Articles by Magee, J. T. Articles by Aries, R. E.

A pyrolysis mass spectrometry study of fusobacteria

J. T. Magee, J. M. Hindmarch^*, K. W. Bennett^*, B. I. Duerden and R. E. Aries

Department of Microbiology, Children's Hospital, Sheffield S10 2TH

^* Department of Bacteriology, Royal Hallamshire Hospital, Sheffield

Department of Medical Microbiology, University of Sheffield

Perkin Elmer Ltd, Beaconsfield, Bucks HP9 1QA

Received July 28, 1988
Accepted September 21, 1988

Strains of fusobacteria (143) were examined by pyrolysis mass spectrometry (Py-MS) with a Horizon Instruments PYMS 200X. Fusobacterium necrogenes, F. necrophorum, F. nucleatum, F. mortiferum, F. varium, F. gonidiaformans, F. naviforme, F. russii and Leptotrichia buccalis were discriminated. Strains of fusobacteria isolated from tropical ulcers, although similar to F. mortiferum in conventional tests, were discriminated from each of these species in Py-MS. Identification of 416 spectra to species level agreed with conventional bacteriological methods in 91·8% of cases, was equivocal in 3·4% and disagreed in 4·8%. Classification based on pyrolysis data resolved groups largely corresponding to the recognised species. However, F. nucleatum strains were divided between two distinct groups. The tropical ulcer strains were resolved as a distinct homogeneous group. Py-MS is a rapid, inexpensive and convenient procedure for characterisation of bacteria, with the capacity for a high throughput of samples, although the initial cost of the apparatus is high.

This article has been cited by other articles:

				V. Hall, G. L. O'Neill, J. T. Magee, and B. I. Duerden Development of Amplified 16S Ribosomal DNA Restriction Analysis for Identification of Actinomyces Species and Comparison with Pyrolysis-Mass Spectrometry and Conventional Biochemical Tests J. Clin. Microbiol., July 1, 1999; 37(7): 2255 - 2261. [Abstract] [Full Text]