The Journal of Medical Microbiology, Vol 28, Issue 1 59-67, Copyright © 1989 by Society for General Microbiology

JOURNAL ARTICLE

Invasion of Vero cells by Salmonella species

P. A. Barrow and M. A. Lovell
Department of Microbiology, AFRC Institute for Animal Health, Houghton Laboratory, Huntingdon, Cambridgeshire.

The invasiveness of Salmonella strains for Vero cells was studied by quantitative bacteriology; the technique was more sensitive than phase contrast microscopy. All of 59 Salmonella strains, of 19 different serotypes, were more invasive than Escherichia coli K12. Three strains of Shigella were as invasive as most of the Salmonella strains whereas 29 strains of E. coli, two of Proteus, three of Klebsiella and one of Serratia were much less invasive. Two Citrobacter strains exhibited intermediate invasiveness. Eleven Salmonella strains were also shown to be invasive in HeLa, int 407, bovine kidney, chick kidney and chick embryonic fibroblast cells. The difference between invasive and non-invasive organisms was apparent irrespective of the numbers of bacteria in contact with Vero cells or the duration of bacteria-cell contact. There was little intracellular multiplication of S. typhimurium in Vero cells. Unlike the situation with Shigella, incubation of Salmonella or Salmonella-cell mixtures at 41 degrees C, 22 degrees C or 0 degree C had little effect on invasiveness. Non-viable Salmonella organisms were non-invasive. Incubation of Vero cells with cholera toxin, dinitrophenol, iodoacetic acid, cytochalasin B or D-mannose did not substantially reduce invasiveness. Virulence-associated plasmids were not essential to invasion by S. typhimurium, S. gallinarum or S. pullorum. Neither somatic antigens nor mannose-sensitive haemagglutinins were essential to the invasiveness of an S. infantis strain, but an additional factor, eliminated by N-methyl, N-nitro, N-nitrosoguanidine mutagenesis did contribute to invasiveness.

This article has been cited by other articles:

				P. Wigley, S. Hulme, L. Rothwell, N. Bumstead, P. Kaiser, and P. Barrow Macrophages Isolated from Chickens Genetically Resistant or Susceptible to Systemic Salmonellosis Show Magnitudinal and Temporal Differential Expression of Cytokines and Chemokines following Salmonella enterica Challenge Infect. Immun., February 1, 2006; 74(2): 1425 - 1430. [Abstract] [Full Text] [PDF]

				T. Whitworth, V. L. Popov, X.-J. Yu, D. H. Walker, and D. H. Bouyer Expression of the Rickettsia prowazekii pld or tlyC Gene in Salmonella enterica Serovar Typhimurium Mediates Phagosomal Escape Infect. Immun., October 1, 2005; 73(10): 6668 - 6673. [Abstract] [Full Text] [PDF]

				C. K. Smith, P. Kaiser, L. Rothwell, T. Humphrey, P. A. Barrow, and M. A. Jones Campylobacter jejuni-Induced Cytokine Responses in Avian Cells Infect. Immun., April 1, 2005; 73(4): 2094 - 2100. [Abstract] [Full Text] [PDF]

				A. K. Turner, L. Z. Barber, P. Wigley, S. Muhammad, M. A. Jones, M. A. Lovell, S. Hulme, and P. A. Barrow Contribution of Proton-Translocating Proteins to the Virulence of Salmonella enterica Serovars Typhimurium, Gallinarum, and Dublin in Chickens and Mice Infect. Immun., June 1, 2003; 71(6): 3392 - 3401. [Abstract] [Full Text] [PDF]

				M. A. Jones, P. Wigley, K. L. Page, S. D. Hulme, and P. A. Barrow Salmonella enterica Serovar Gallinarum Requires the Salmonella Pathogenicity Island 2 Type III Secretion System but Not the Salmonella Pathogenicity Island 1 Type III Secretion System for Virulence in Chickens Infect. Immun., September 1, 2001; 69(9): 5471 - 5476. [Abstract] [Full Text] [PDF]

				P. Kaiser, L. Rothwell, E. E. Galyov, P. A. Barrow, J. Burnside, and P. Wigley Differential cytokine expression in avian cells in response to invasion by Salmonella typhimurium, Salmonella enteritidis and Salmonella gallinarum Microbiology, December 1, 2000; 146(12): 3217 - 3226. [Abstract] [Full Text] [PDF]