The Journal of Medical Microbiology, Vol 27, Issue 4 227-232, Copyright © 1988 by Society for General Microbiology

JOURNAL ARTICLE

Characterisation and cellular localisation of the immunodominant 47-Kda antigen of Candida albicans

R. Matthews, C. Wells and J. P. Burnie
Department of Medical Microbiology, St Bartholomew's Hospital, West Smithfield, London.

The 47-Kda component of Candida albicans is an immunodominant antigen in the serology of systemic candidosis. Immuno-electronmicroscopy with an affinity-purified antibody to the 47-Kda antigen showed that it was present in the cytoplasm and cell wall of both yeast and mycelial cells. It was found in discrete areas on the inner and outer borders of the cell wall and was mainly located within the wall rather than exposed on the outer surface. Sometimes it appeared to be in channels across the cell wall. In the cytoplasm, it was usually near the cytoplasmic membrane and occasionally appeared in vesicular areas. It was not detected in the nucleus or mitochondria. The 47-Kda antigen did not bind to Concanavalin A, and antigenicity was lost after protease digestion. Peptide mapping suggested that the antigen was highly conserved between different strains of C. albicans.

This article has been cited by other articles:

				N. Denikus, F. Orfaniotou, G. Wulf, P. F. Lehmann, M. Monod, and U. Reichard Fungal Antigens Expressed during Invasive Aspergillosis Infect. Immun., August 1, 2005; 73(8): 4704 - 4713. [Abstract] [Full Text] [PDF]

				R. C. Matthews, G. Rigg, S. Hodgetts, T. Carter, C. Chapman, C. Gregory, C. Illidge, and J. Burnie Preclinical Assessment of the Efficacy of Mycograb, a Human Recombinant Antibody against Fungal HSP90 Antimicrob. Agents Chemother., July 1, 2003; 47(7): 2208 - 2216. [Abstract] [Full Text] [PDF]

				E. T. Burt, R. Daly, D. Hoganson, Y. Tsirulnikov, M. Essmann, and B. Larsen Isolation and Partial Characterization of Hsp90 from Candida albicans Ann. Clin. Lab. Sci., January 1, 2003; 33(1): 86 - 93. [Abstract] [Full Text] [PDF]

				A. Pitarch, M. Sanchez, C. Nombela, and C. Gil Sequential Fractionation and Two-dimensional Gel Analysis Unravels the Complexity of the Dimorphic Fungus Candida albicans Cell Wall Proteome Mol. Cell. Proteomics, December 1, 2002; 1(12): 967 - 982. [Abstract] [Full Text] [PDF]

				A. Viudes, S. Perea, and J. L. Lopez-Ribot Identification of Continuous B-Cell Epitopes on the Protein Moiety of the 58-Kilodalton Cell Wall Mannoprotein of Candida albicans Belonging to a Family of Immunodominant Fungal Antigens Infect. Immun., May 1, 2001; 69(5): 2909 - 2919. [Abstract] [Full Text] [PDF]

				W. L. Chaffin, J. L. Lopez-Ribot, M. Casanova, D. Gozalbo, and J. P. Martinez Cell Wall and Secreted Proteins of Candida albicans: Identification, Function, and Expression Microbiol. Mol. Biol. Rev., March 1, 1998; 62(1): 130 - 180. [Abstract] [Full Text] [PDF]

				J. P. Martinez, M. L. Gil, J. L. Lopez-Ribot, and W. L. Chaffin Serologic Response to Cell Wall Mannoproteins and Proteins of Candida albicans Clin. Microbiol. Rev., January 1, 1998; 11(1): 121 - 141. [Abstract] [Full Text] [PDF]