J Med Microbiol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shand, G. H.
Right arrow Articles by Hoiby, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shand, G. H.
Right arrow Articles by Hoiby, N.
Agricola
Right arrow Articles by Shand, G. H.
Right arrow Articles by Hoiby, N.

The Journal of Medical Microbiology, Vol 27, Issue 3 169-177, Copyright © 1988 by Society for General Microbiology

JOURNAL ARTICLE

Use of immunoblot detection of serum antibodies in the diagnosis of chronic Pseudomonas aeruginosa lung infection in cystic fibrosis

G. H. Shand, S. S. Pedersen, R. Tilling, M. R. Brown and N. Hoiby
Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.

Antibodies to Pseudomonas aeruginosa were investigated in serum from cystic fibrosis (CF) patients by immunoblotting (Western blotting). The results were compared with determinations of precipitating antibodies in serum by crossed immune electrophoresis (CIE). The number of CIE precipitins is a sensitive and specific indication of infection and is used, with sputum bacteriology, to distinguish between colonisation and invasive lung infection. Immunoblotting was considerably more sensitive than CIE for detecting antibodies to P. aeruginosa. Paired serum samples from 64 CF patients, taken before a diagnosis of P. aeruginosa lung infection and immediately afterwards, showed a marked increase in the number of serum antibodies with the onset of infection. The intensity of the reaction, as shown by the density of blotted bands, was also increased. Laser scanning densitometry of immunoblots, and of photographic negatives taken from them, was used to quantify the increases. Differences in the number and intensity of blotted bands were highly significant between the two groups. The reproducibility of the method was good. An immunoblot assay may be a sensitive and useful method for routine diagnosis of early P. aeruginosa lung infection in CF.


This article has been cited by other articles:


Home page
 ThoraxHome page
E Caballero, M-E Drobnic, M-T Perez, J-M Manresa, A Ferrer, and R Orriols
Anti-Pseudomonas aeruginosa antibody detection in patients with bronchiectasis without cystic fibrosis
Thorax, September 1, 2001; 56(9): 669 - 674.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
G. Pier, G. Small, and H. Warren
Protection against mucoid Pseudomonas aeruginosa in rodent models of endobronchial infections
Science, August 3, 1990; 249(4968): 537 - 540.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 1988 Society for General Microbiology.