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The Journal of Medical Microbiology, Vol 27, Issue 2 141-144, Copyright © 1988 by Society for General Microbiology

JOURNAL ARTICLE

Use of enzymes in typing group A beta-haemolytic streptococci

K. N. Brahmadathan and G. Koshi
Department of Microbiology, Christian Medical College Hospital, Vellore, Tamil Nadu, India.

A series of 698 strains of group A beta-haemolytic streptococci (GAS) isolated from children with streptococcal pyoderma was tested for production of serum opacity factor (OF) and nicotinamide adenine dinucleotide glycohydrolase (NADase). OF was produced by 37% of strains and 40% produced NADase. Classification based on various combinations of OF and NADase reactions showed that 58% belonged to enzyme group el and 34% to e2. Correlation with T and M types showed the possible use of this means of classification as an epidemiological marker for GAS. The specificity of such a system in the further classification of various T types of GAS in epidemiological studies, in the light of antigenic variation among M types, is described.




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