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The Journal of Medical Microbiology, Vol 25, Issue 3 187-190, Copyright © 1988 by Society for General Microbiology

JOURNAL ARTICLE

Production of monoclonal antibody against Aeromonas hydrophila haemolysin

S. Kozaki, K. Kato, A. Kurokawa, Y. Kamata, T. Asao and G. Sakaguchi
Department of Veterinary Science, College of Agriculture, University of Osaka Prefecture, Japan.

Two hybridoma cell lines that produce monoclonal antibodies against Aeromonas hydrophila haemolysin were established by fusion of myeloma and spleen cells obtained from a mouse immunised with haemolysin detoxified with tetranitromethane. Enzyme-linked immunosorbent assay (ELISA) showed that the two purified monoclonal antibodies, B7 and B11, recognised the same epitope on the haemolysin molecule. Antibody B7 neutralised the haemolytic and enterotoxic activities of the haemolysin. It is concluded that the same site on the haemolysin molecule is responsible for both haemolytic and enterotoxic activities.




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