The Journal of Medical Microbiology, Vol 24, Issue 2 133-137, Copyright © 1987 by Society for General Microbiology
Identification of Bacteroides species from adult periodontal disease
B. I. Duerden, L. Goodwin and T. C. O'Neil
Department of Medical Microbiology, University of Sheffield Medical School.
Samples from deep (4-7 mm) periodontal pockets were collected from 17
patients with adult-type periodontal disease and one with the juvenile form
of the disease. They were streaked immediately on selective and
non-selective media and incubated anaerobically for 96 h. There was a heavy
growth of Bacteroides spp. from most samples and 10 representative colonies
from each sample were sub-cultured for identification. In a total of 149
isolates from patients with adult-type disease, the commonest species were
B. oralis (40), B. asaccharolyticus (35), B. intermedius (31), B. fragilis
(12) and B. ureolyticus (10); B. gingivalis was not detected. The
distribution of species was not distorted by multiple identical isolates
from individual patients. There was a heavy growth of a single species, B.
ureolyticus, from the patient with juvenile-type disease.
