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The Journal of Medical Microbiology, Vol 24, Issue 2 113-118, Copyright © 1987 by Society for General Microbiology


JOURNAL ARTICLE

Typing of Aeromonas species by polyacrylamide-gel electrophoresis of radiolabelled cell proteins

J. R. Stephenson, S. E. Millership and S. Tabaqchali
Department of Medical Microbiology, St Bartholomew's Hospital Medical College, London.

A method for typing species of Aeromonas by 35S-labelling and sodium-dodecyl sulphate polyacrylamide-gel electrophoresis (SDS-PAGE) of total soluble proteins is described. Radiolabelled-protein profiles from different strains were compared by use of the Dice coefficient. Typability was 100% and discrimination of pairs of differing strains was satisfactory with acceptable reproducibility. The results also yielded information on subgroups of phenotypic species.





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