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The Journal of Medical Microbiology, Vol 23, Issue 3 227-232, Copyright © 1987 by Society for General Microbiology

JOURNAL ARTICLE

Studies on the Vibrio cholerae mucinase complex. II. Specific neuraminidase activity measured histochemically in a goblet cell assay

R. A. Ollar and D. E. Stewart-Tull

The activity of neuraminidase prepared from the mucinase complex of Vibrio cholerae was measured by a new, semi-quantitative goblet-cell assay. The counts of normal, alcianophilic, sialomucin-containing goblet cells (purple-stained) and neutral mucosubstance-containing goblet cells (magenta-stained) in serial sections of ileum were compared before and after neuraminidase treatment. The procedure provides a more natural assessment of the action of V. cholerae neuraminidase on the viscous intestinal mucus.




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