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The Journal of Medical Microbiology, Vol 22, Issue 4 315-317, Copyright © 1986 by Society for General Microbiology

JOURNAL ARTICLE

Comparison of direct plating with the use of enrichment culture for isolation of Aeromonas spp. from faeces

J. Robinson, J. Beaman, L. Wagener and V. Burke

Direct plating of faecal specimens on blood agar was compared with the use of enrichment culture for isolation of Aeromonas spp. from faeces during a large epidemiological study. Of enterotoxigenic strains isolated by direct plating, 89% were associated with acute diarrhoea and 7% with an episode of diarrhoea during the month before collection, but 79% of enterotoxigenic strains isolated only after enrichment were not associated with acute diarrhoea. With Aeromonas spp., as with intestinal pathogens, it appears that enrichment allows isolation of the bacteria when in low faecal concentrations likely to be found in convalescent patients, carriers and those with subclinical infection. The routine use of enrichment for isolation of faecal aeromonads, by detecting Aeromonas spp. in low numbers in patients without diarrhoea, is likely to confuse interpretation of epidemiological studies seeking to clarify the relationship between Aeromonas spp. and acute diarrhoea.


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A. Andelova, I. Porazilova, and E. Krejci
Aeromonas agar is a useful selective medium for isolating aeromonads from faecal samples.
J. Med. Microbiol., November 1, 2006; 55(Pt 11): 1605 - 1606.
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Copyright © 1986 Society for General Microbiology.