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The Journal of Medical Microbiology, Vol 21, Issue 4 343-347, Copyright © 1986 by Society for General Microbiology

JOURNAL ARTICLE

Staphylococcus aureus protein A--antibody-mediated haemagglutination for the analysis of cross-reactivity between phenol-water extracts of Bacteroides fragilis

N. Srinivasakumar and R. S. Rao

Passive haemagglutination (PHA) and Staphylococcus aureus protein A--antibody-mediated haemagglutination (SAPA-AMHA) were used to analyse the cross-reactions of rabbit antisera against four strains of Bacteroides fragilis. There was cross-reactivity between all the strains tested but strain-specific reactions were obtained with three strains. Two to 32-fold higher antibody titres were obtained with SAPA-AMHA than with PHA. The antigen concentration required in inhibition assays was up to 32-fold higher for PHA than for SAPA-AMHA. The latter is a simple and superior alternative to PHA for such studies.




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