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The Journal of Medical Microbiology, Vol 21, Issue 3 215-218, Copyright © 1986 by Society for General Microbiology

JOURNAL ARTICLE

Rapid identification of gram-negative bacilli from blood cultures

M. Stevens and G. T. Parish

Blood-culture broths showing macroscopic or radiometric evidence of growth of gram-negative bacilli were examined by a rapid automated bacterial identification system. A differential centrifugation technique was developed to prepare suitable inocula. The identification results obtained were confirmed by the API 20E method, with single colonies of the strains isolated 24 h later. Of 90 organisms tested, seven did not give the same identification by the two systems. With the rapid automated technique a presumptive identification of gram-negative bacilli can be made 24 h earlier than by more conventional methods.




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