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J Med Microbiol 21 (1986), 145-150; DOI: 10.1099/00222615-21-2-145
© 1986 Society for General Microbiology
ISSN 0022-2615
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A collaborative evaluation of a rapid, semi-automated identification system for gram-negative bacilli: the Quantum II BID

M. Stevens2, C. Henrichsen* and M. Smith*

*Department of Bacteriology, Edinburgh University, Teviot Place, Edinburgh EH8 9AG, Scotland

1Public Health Laboratory, Royal Infirmary, Leicester LE1 5WW, England

Received November 20, 1984
Accepted May 21, 1985

The recently-introduced semi-automated bacterial identification system, the Quantum II BID, is designed to identify fermentative and non-fermentative gram-negative bacilli in 4–5 h. The system was evaluated independently by the two participating laboratories. Inter-laboratory reproducibility was determined by testing 181 strains in each laboratory and found to be 97·8%. A further 893 organisms, comprising 417 fresh clinical isolates and 476 stock cultures, were then tested by the system in the two laboratories. Of the fresh clinical isolates, 95·7% yielded the same result as the comparative system used (API 20E) and 93·1% of the stock strains were assigned to their expected, previously established identity. Overall agreement between the two systems for all strains examined was 94·3%. There was no significant statistical difference between the results obtained in the two laboratories.







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Copyright © 1986 Society for General Microbiology.