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The Journal of Medical Microbiology, Vol 21, Issue 1 87-90, Copyright © 1986 by Society for General Microbiology

JOURNAL ARTICLE

Characterisation of mouse monoclonal antibodies produced by immunisation with a single serotype component of a polyvalent Pseudomonas aeruginosa vaccine

G. R. Barclay, P. L. Yap, D. B. McClelland, R. J. Jones, E. A. Roe, M. C. McCann, L. R. Micklem and K. James

Mouse monoclonal antibodies raised by immunisation with a protective antigen extract from Pseudomonas aeruginosa serotype 1 varied in immunoglobulin isotype, in passive protective properties against infection by homologous P. aeruginosa serotype 1, and in cross-reactions in ELISA against antigen preparations from 15 other P. aeruginosa serotypes. All monoclonal antibodies with specificity in ELISA for the immunising antigen gave some degree of protection to mice against lethal infection by the homologous P. aeruginosa serotype. The IgG antibodies were more protective than the IgM antibodies.


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Y. Han, M. H. Riesselman, and J. E. Cutler
Protection against Candidiasis by an Immunoglobulin G3 (IgG3) Monoclonal Antibody Specific for the Same Mannotriose as an IgM Protective Antibody
Infect. Immun., March 1, 2000; 68(3): 1649 - 1654.
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