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The Journal of Medical Microbiology, Vol 20, Issue 3 299-305, Copyright © 1985 by Society for General Microbiology


JOURNAL ARTICLE

Role of the fungal cell wall in the granulomatous response of mice to the agents of chromomycosis

C. L. Silva and R. A. Fazioli

Mice were given, intraperitoneally, inocula of a cell-wall preparation and fractions thereof from Fonsecaea pedrosoi, F. compactum, Cladosporium carrioni and Phialophora verrucosum. Large doses of cell-wall preparation, with or without trypsin treatment, produced a pronounced loss of body weight, a granulomatous reaction and, sometimes, death. After extraction of the cell wall preparation with 1N NaOH, three fractions were obtained: an alkali-insoluble fraction 1; an alkali-soluble acid-insoluble fraction 2; and an alkali- and acid-soluble fraction 3. Intravenous administration showed that only fraction 1 induced a granulomatous reaction and death in mice.


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