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The Journal of Medical Microbiology, Vol 20, Issue 2 263-274, Copyright © 1985 by Society for General Microbiology

JOURNAL ARTICLE

Immunological responses to protein, carbohydrate and lipid fractions of Nocardia asteroides in mice

R. Gupta, V. Pancholi, V. K. Vinayak and G. K. Khuller

The protein, polysaccharide and phospholipid constituents of Nocardia asteroides have been partially purified and their immunogenicity studied in mice. Humoral and cellular immune responses were demonstrated against a crude cytoplasmic protein fraction (CPF). Two fractions of CPF were prepared on a Sephadex G-200 column; a high mol. wt fraction, fraction-1(F1) was capable of eliciting both types of immune responses, whereas fraction-2(F2) behaved more like a hapten. Phosphatides elicited only humoral responses whereas polysaccharides were non-immunogenic.




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Copyright © 1985 Society for General Microbiology.