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The Journal of Medical Microbiology, Vol 17, Issue 3 295-310, Copyright © 1984 by Society for General Microbiology


JOURNAL ARTICLE

Antibiotic susceptibilities and plasmid profiles of nosocomial methicillin-resistant Staphylococcus aureus: a retrospective study

M. T. Gillespie, J. W. May and R. A. Skurray

Methicillin-resistant Staphylococcus aureus strains isolated at a single Melbourne Hospital between 1969 and 1981 were examined for susceptibility to a range of antimicrobial agents and for the presence of plasmid DNA. Isolates obtained during 1969 possessed a plasmid of mol. wt 20 X 10(6), encoding heavy metal resistance and penicillinase production, and a plasmid of mol. wt 2.8 X 10(6), mediating tetracycline resistance. In the majority of isolates obtained after 1973, these functions were chromosomally encoded. Before 1980, both high- and low-level chromosomally-encoded gentamicin resistances were encountered, whereas isolates from 1980 and 1981 displayed low-level gentamicin resistance only; the latter phenotype was most commonly mediated by a plasmid of mol. wt 18 X 10(6) that also encoded resistance to tobramycin and kanamycin. Chloramphenicol resistance in strains isolated throughout the period was mediated by one of three plasmids, each of mol. wt c. 3 X 10(6).


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S. A. Khan, M. S. Nawaz, A. A. Khan, and C. E. Cerniglia
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