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The Journal of Medical Microbiology, Vol 15, Issue 4 493-501, Copyright © 1982 by Society for General Microbiology

JOURNAL ARTICLE

Immunogenicity of leptospiral vaccines grown in protein-free medium

W. L. Christopher, B. Adler and S. Faine

Leptospira interrogans serovars pomona and hardjo were adapted to grow in a chemically-defined, protein-free (PF) medium. Formolised monovalent vaccines of serovars pomona and hardjo and a bivalent mixture of the two were prepared from PF cultures. Live PF cultures and the vaccine preparations retained their agglutinating antigens and their immunogenicity when tested in rabbits and guinea-pigs. The vaccines were not pyrogenic and dermal reactions were slight.


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