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The Journal of Medical Microbiology, Vol 13, Issue 4 573-579, Copyright © 1980 by Society for General Microbiology


JOURNAL ARTICLE

The carbon dioxide requirements of anaerobic bacteria

S. Reilly

Some clinically significant anaerobic bacteria were incubated in pure culture in anaerobic jars containing a range of atmospheric concentrations of CO2. Growth of Clostridium perfringens, C. sporogenes and C. septicum was independent of the amount of CO2. Small supplements of CO2 (0.25%) allowed good growth of the majority of anaerobes studied--an observation contrary to established teaching. The exceptions were Fusobacterium necrophorum which showed an absolute requirement for CO2 of at least 1% and B. melaninogenicus which needed an atmospheric content of 10--40% CO2 for optimal growth. The inclusion of CO2 in the anaerobic jar at a final concentration of 10% is to be recommended for all routine isolation procedures.





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