The Journal of Medical Microbiology, Vol 13, Issue 2 213-221, Copyright © 1980 by Society for General Microbiology
Immunogenicity and molecular characterisation of staphylococcal delta haemolysin
T. H. Birkbeck and D. D. Whitelaw
In rabbit antisera to staphylococcal delta haemolysin the
haemolysin-neutralising activity was associated with the IgG fraction as
well as with the alpha and beta lipoproteins. On an equal-weight basis, the
neutralising capacity of specific antibody was not significantly greater
than that of serum alpha or beta lipoproteins. Anti-alpha haemolysin was
not detected in any of the anti-delta-haemolysin antisera. Amino-acid
analysis of delta haemolysin showed that lysine, aspartic acid, threonine
and isoleucine were the predominant amino acids present; histidine,
arginine, proline, cysteine and tyrosine were absent. The molecular weight
of delta-haemolysin in phosphate-buffered saline was approximately 210 000.
