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The Journal of Medical Microbiology, Vol 13, Issue 1 163-166, Copyright © 1980 by Society for General Microbiology

JOURNAL ARTICLE

Isolation of Dane particles containing a DNA strand by metrizamide density gradient

T. Takahashi, K. Kaga, Y. Akahane, T. Yamashita, Y. Miyakawa and M. Mayumi

Dane-particle cores labelled with [3H]TTP were subjected to ultracentrifugation in a metrizamide density gradient. Two populations of core particles with different densities were obtained, and radioactivity was found only in the cores that sedimented at the lower density (1.19-1.23 g/cm3). All the cores in this group, when spread in a monolayer, were found to expel a closed circular double-stranded DNA molecule. In contrast, the core particles that sedimented at the higher density (1.23-1.27 g/cm3) were not associated with radioactivity, nor was any DNA strand extruded from them. These results show that metrizamide density gradients allow the separation of complete hepatitis B virions for the study of viral DNA.


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T. Kimura, N. Ohno, N. Terada, A. Rokuhara, A. Matsumoto, S. Yagi, E. Tanaka, K. Kiyosawa, S. Ohno, and N. Maki
Hepatitis B Virus DNA-negative Dane Particles Lack Core Protein but Contain a 22-kDa Precore Protein without C-terminal Arginine-rich Domain
J. Biol. Chem., June 10, 2005; 280(23): 21713 - 21719.
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