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The Journal of Medical Microbiology, Vol 11, Issue 4 541-545, Copyright © 1978 by Society for General Microbiology

JOURNAL ARTICLE

A simple and safe volumetric alternative to the method of Miles, Misra and Irwin for counting viable bacteria

M. P. Slack and D. B. Wheldon

A rapid and simple method for counting viable bacteria is described. The technique involves the use of a semi-automatic micropipette and disposable glass-capillary tubes. Viable counts obtained by this method were not significantly different from those obtained by the Miles, Misra and Irwin method or by a pour-plate method. The micropipette procedure is less hazardous than techniques involving the use of calibrated dropping pipettes.




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