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J Med Microbiol 11 (1978), 315-324; DOI: 10.1099/00222615-11-3-315
© 1978 Society for General Microbiology
ISSN 0022-2615
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The Use of Fluorescein-Labelled Lectins in the Detection and Identification of Fungi Pathogenic for Man: A Preliminary Study

R. W. STODDART* and B. M. HERBERTSON{dagger}

* Strangeways Research Laboratory, Worts' Causeway, Cambridge CB1 4RN

{dagger} Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP

Received November 2, 1977
Accepted January 17, 1978

Fluorescein-labelled lectins of known specificities for different sugars were used in an attempt to identify fungi in paraffin sections of surgical and postmortem material. Aspergillus fumigatus, Blastomyces dermatitidis, Candida albicans, Cryptococcus neoformans, Paracoccidioides brasiliensis and Rhizopus oryzae have been studied with five fluorescein-labelled lectins and with fluorescein-labelled aprotinin. The fungi were readily distinguishable on the basis of differences in their reactions with these stains. The results accord well with what is known of the chemistry of the organisms and the method offers promise to practising histopathologists.




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