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Assay of Staphylococcal -Haemolysin with Fish Erythrocytes

Department of Microbiology, University of Glasgow, Alexander Stone Building, Garscube Estate, Bearsden, Glasgow G61 1QH

Received October 17, 1977 Revision received January 9, 1978.
Accepted January 9, 1978

Suspensions of erythrocytes from certain marine fish (cod, saithe, haddock and mackerel) were 4-16 times more sensitive than human or horse erythrocytes to staphylococcal -haemolysin. Cod erythrocytes were lysed rapidly by -haemolysin and the titres of haemolysin were independent of the temperatures at which the tests were incubated, over the range 0-37°C. Fish erythrocytes were not lysed by purified - and β-haemolysins and no synergistic action was found between β- and -haemolysins. In blood agar-overlay plates, the zones of lysis that developed in cod blood agar were larger and clearer than those that developed in human or horse blood agar.