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A method for the isolation of Bacteroides melaninogenicus from the human mouth

W. P. HOLBROOK^*,, S. A. OGSTON and P. W. ROSS^*

^*Department of Bacteriology, University Medical School, Teviot Place, Edinburgh EH8 9AG

Department of Statistics, The King's Buildings, University of Edinburgh, Edinburgh EH9 3JZ

Received October 5, 1977
Accepted October 28, 1977

Isolation procedures involving the direct plating of specimens and the use of selective, non-selective, transport and enrichment media were compared in respect of their value for the recovery of Bacteroides melaninogenicus from the gingival sulcus. A selective medium containing kanamycin and vancomycin enhanced the recovery rate of B. melaninogenicus. VMGII transport medium was convenient to use and gave as good a recovery rate as that obtained with plates directly inoculated in the clinic.

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