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Comparison of several culture media used for studies on mycobacteriophages (Plates XII–XIV)

C. E. STAGER^* and P. R. J. GANGADHARAM^,

^*Mycobacteriology Laboratories, Jefferson Davis Hospital, Baylor College of Medicine, Houston, Texas 77025, USA

Department of Pathology, Baylor College of Medicine, Houston, Texas 77025, USA

Received September 17, 1976
Accepted October 29, 1976

The value of RVA, N-l, 7H10, 7H11 and Sauton's media for studies on mycobacterio-phage infection and lysis of mycobacteria was assessed. Experiments were made with mycobacteriophages BGI, BKI, CRI-3, G37, and LG, all of which lyse Mycobacterium smegmatis strain 607B, and with mycobacteriophage DS6A which lyses Mycobacterium tuberculosis strain H37Rv. The methods involved "direct lysis", the measurement of "routine test dilutions" and counts of plaque-forming units. It was found that N-l, 7H10 and 7H11 media gave better overall results than RVA medium for M. smegmatis strain 607B and its phages, and that RVA medium was generally the most useful for M. tuberculosis strain H37Rv and its phage. Sauton's medium was inadequate for all the phage-host systems employed.