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The Journal of Medical Microbiology, Vol 10, Issue 2 249-253, Copyright © 1977 by Society for General Microbiology

JOURNAL ARTICLE

Delayed culture of group-A streptococci: an evaluation of variables in methods of examining throat swabs

D. R. Martin, J. M. Stanhope and L. A. Finch

We studied the effects on the frequency of isolation of group-A streptococci from throat swabs of school children on three varibles: (1) plating the cultures within 4 h of collecting the swabs or after storage for 4 days at room temperature; (2) using a plain or a serum-coated cotton swab; and (3) including silica gel in the swab tube or omitting this. Under the conditions of our experiment, only delayed plating of the swab gave a statistically significant advantage. When the delayed-isolation technique was used, neither the swab type nor the presence or absence of silica gel significantly influenced the result. The least advantageous combination of variables was: plating within 4 h, a plain swab, and the absence of silica gel.


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M. McDonald, R. Towers, P. Fagan, M. McKinnon, N. Benger, R. Andrews, B. J. Currie, and J. Carapetis
Recovering Streptococci from the Throat, a Practical Alternative to Direct Plating in Remote Tropical Communities
J. Clin. Microbiol., February 1, 2006; 44(2): 547 - 552.
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